The auto-inhibitory role of the EPAC hinge helix as mapped by NMR.
The cyclic-AMP binding domain (CBD) is the central regulatory unit of exchange proteins activated by cAMP (EPAC).The CBD maintains EPAC in a state of auto-inhibition in the absence of the allosteric effector, Frozen Beverage Machines cAMP.When cAMP binds to the CBD such auto-inhibition is released, leading to EPAC activation.It has been shown that